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Q1. What’s included in the microbial mNGS localization solution?
The microbial mNGS localization solution include Microbial homogenizer, NGSmaster, Cartridge, Reagent kits, Data analysis software and server.
Q2. What’s kind of sequencing platform is compatible with?
Hiseq, Miseq, Minseq, Nextseq from illumina.
Q3. How long does it take from sample to report for the microbial mNGS solution?
It can finish the whole process of mNGS(from sample to report) at 13 hours.
Q4. What’s the throughput for NGSmaster?
Each standard NGSmaster has four channels, each channel is independent, can prep the libraries in different type at the same time. The NGSmaster can also be multipied on needs, maximum 24units of NGSmaster with four channels(96 channels)
The one channel NGSmaster is also available.
Q5. What’s type of library which NGSmaster can prepare, how long does it take?
GSmaster can make the DNA library preparation and RNA library preparation.
Samples in, libraries out, full automation. For DNA library, it will take 2 hours, for RNA library, it will take 3.5 hours.
Q6. Why it only takes 2 hours for DNA library preparation?
The conventional library prep method will take 6 to 8 hours. (PCR library prep by manual). MatriDx uses the PCR-free technology, during the processes, it doesn’t make PCR amplification(five steps for library prep: Nucleic acid extracting, Fragmentation, End repair and A tailing, Adaptor ligation, Purification). with the automation of NGSmaster, the turnaround time can be reduced at 2 hours.
Q7. Why MatriDx uses the PCR-free technology, does it have any advantages?
1) The PCR-free technology does’t have the process of PCR amplification, it has no risk of PCR aerosol contamination
2) No PCR bias, Results are more real.
3) The process is more simple for PCR-free technology, the turnaround time is more short.
Q8. What’s the sample type which can be prepared by NGSmaster?
The samples type includes: Plasma, Bone marrow, BALF, CSF, Hydrothorax and ascites, Bile, Hydrarthrosis, Urine, Abscess fluid, Human aquenous, other puncture fluid, Tissue, Sputum, Nasopharyngeal swab, Wound exudates.
Q9. how the make the quality control for the library?
After getting the Library by NGSmaster, use the Real-time PCR to qualify the library, and then pooling. Quality inspection after pooling, then sequencing.
Q10. what about the size of NGSmaster, is it any requirement for space?
NGSmaster is quite small when compare with the other autoamted library prep instrument, the size is 518mm*343mm*418mm. It can be put into a biological safety cabinet, biological safety is guaranteed. It doesn’t have to put it in the PCR room.
Q11. How to make the data analysis and how to issue the report
MatriDx provides the Data analysis software, it includes three parts: GentellixTM Intelligent Analysis System, MicroBaseTM Species Information System, DeterpretTM Depth Interpretation System. The data analysis software can meet the needs of labs to carry out automatic real-time quality control and analysis of sequencing data during the mNGS processing, One-click to general a report, rapid, user friendly and high security, it assists physicians to precise diagnosis and treatment decisions for patients.
MatriDx provides local server data analysis solution and cloud server solution for option.
Q12. What’s kind of microorganism can be detected?
MatriDx microbial mNGS can make the detection for 24419 kinds of microorganism which includes Bacteria,Virus, Parasite, Fungi and more than 2000 antibiotic resistance genomes.
Q13. What’s the advantages of MatriDx’s localization microbial mNGS solution?
1）the microbial Homogenizer significantly increase the detection rate of difficult-to-break microorganisms such as staging bacilli and fungi without affecting the detection of other microorganisms
2) NGSmaster automated library prep instrument can make the nucleic acid extraction and Library preparation rapidly at 2 hours, it short the turnaround time, eliminate the contamination, guarantee the biological safety.
3) Uses PCR-free technology, significantly short the turnaround time, avoid PCR bias, make the detection results more real.
4) Analysis software includes GentellixTM Intelligent Analysis System, MicroBaseTM Species Information System and DeterpretTM Depth Interpretation System, generate a report with one button and utmost to complete the report from Sequence data in 10 minutes .